Profiling of Salivary Exosomal Micro RNAs in Burning Mouth Syndrome Patients
김균요, 변진석, 정재광, 최재갑,
소속 상세정보
김균요 ( Kim Kyun-Yo ) - Kyungpook National University School of Dentistry Department of Oral Medicine
변진석 ( Byun Jin-Seok ) - Kyungpook National University School of Dentistry Department of Oral Medicine
정재광 ( Jung Jae-Kwang ) - Kyungpook National University School of Dentistry Department of Oral Medicine
최재갑 ( Choi Jae-Kap ) - Kyungpook National University School of Dentistry Department of Oral Medicine
Abstract
Purpose: The exact causes of burning mouth syndrome (BMS) is unclear so far. There are many studies to elucidate the relation between oral disease and genetic predisposition. In this study, we first tried to investigate salivary exosomal genetic components that could play an important role for diagnosing and elucidating the progression of BMS.
Methods: We compared salivary exosomal micro RNAs (miRNAs) of BMS Patients to those of control using next generation sequencing (NGS). Unstimulated whole saliva from 15 patients with BMS and 10 control subjects were divided into two sets. Isolated exosomes and their total RNAs were subject to NGS for the screening of miRNAs.
Results: There were up-regulated 10 exosomal miRNAs (hsa-miR-1273h-5p, hsa-miR-1273a, hsa-miR-1304-3p, hsa-miR-4449, hsa-miR-1285-3p, hsa-miR-6802-5p, hsa-miR-1268a, hsa-miR-1273d, hsa-miR-1273f, and hsa-miR-423-5p) and down-regulated 18 exosomal miRNAs (hsa-miR-27b-3p, hsa-miR-16-5p, hsa-miR-186-5p, hsa-miR-142-3p, hsamiR-141-3p, hsa-miR-150-5p, hsa-miR-374a-5p, hsa-miR-93-5p, hsa-miR-29c-3p, hsamiR-29a-3p, hsa-miR-148a-3p, hsa-miR-22-3p, hsa-miR-27a-3p, hsa-miR-424-5p, hsamiR-19b-3p, hsa-miR-99a-5p, hsa-miR-548d-3p, and hsa-miR-19a-3p) in BMS patients comparing with those of control subjects.
Conclusions: We show that there are 28 differential expression of miRNAs between the patients with BMS and those of control subjects. The specific function of indicated miRNAs should be further elucidated.
키워드
Burning mouth syndrome; miRNA; Mi-RNAs; Next generation sequencing
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